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cell culture c2c12 mouse myoblasts  (ATCC)


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    ATCC cell culture c2c12 mouse myoblasts
    Cell Culture C2c12 Mouse Myoblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8596 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+mouse+c2c12+myoblast+cells/C2C12/pm42019598-61-5-11
    Average 99 stars, based on 8596 article reviews
    cell culture c2c12 mouse myoblasts - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: Nuclear m6A reader YTHDC1 promotes muscle stem cell activation/proliferation by regulating mRNA splicing and nuclear export.
    Article Snippet: .. Cell lines and cell culture Mouse C2C12 myoblast cells (CRL- 1772) and 293T cells (CRL- 3216) were obtained from American Type Culture Collection (ATCC) and cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml of penicillin, and 100 μg of streptomycin (growth medium [GM]) at 37°C in 5% CO2. ..

    Article Title: Skeletal Muscle Stem Cells Modulate Niche Function in Duchenne Muscular Dystrophy through YY1-CCL5 Axis
    Article Snippet: For treadmill test, mice were adapted to a treadmill (Panlab, Harvard Apparatus, 76-0895) with a 5° incline at an initial speed of 10 cm/s, followed by a stepwise increase of 5 cm/s every two min until their exhaustion. .. Cell lines and cell culture Mouse C2C12 myoblast cells (CRL-1772) and 293T cells (CRL-3216) were obtained from American Type Culture Collection (ATCC) and cultured in DMEM medium (Gibco, 12800-017) with 10% fetal bovine serum (Invitrogen, 16000044), 100 units/ml of penicillin and 100 μg of streptomycin (P/S, 24 Gibco,15140-122) at 37 °C in 5% CO2. ..

    Article Title: Skeletal muscle stem cells modulate niche function in Duchenne muscular dystrophy mouse through YY1-CCL5 axis.
    Article Snippet: .. Cell lines and cell culture Mouse C2C12 myoblast cells (CRL-1772) and 293T cells (CRL-3216) were obtained from American Type Culture Collection and cultured in DMEM medium (Gibco, 12800-017) with 10% fetal bovine serum (Invitrogen, 16000044), 100 units/ml of penicillin and 100 μg of streptomycin (P/S, Gibco,15140-122) at 37 °C in 5% CO2. ..

    Article Title: Injectable gel graft for bone defect repair
    Article Snippet: .. Cell culture Mouse C2C12 myoblast cells (American Type Culture Collection [ATCC], VA, USA) and mouse NIH 3T3 fibroblast cells (ATCC) were cultured in high glucose DMEM (Mediatech, VA, USA) supplemented with 10% (v/v) fetal bovine serum (Lonza, MD, USA) and 1% (v/v) penicillin–streptomycin (Mediatech) at 37°C in a humidified atmosphere of 5% CO 2 . ..

    Article Title: Maduramicin inactivation of Akt impairs autophagic flux leading to accumulated autophagosomes-dependent apoptosis in skeletal myoblast cells
    Article Snippet: .. Cell culture Mouse C2C12 myoblast cells (C2C12 cells) (#CRL-1772) and rat L6 myoblast cells (L6 cells) (#CRL-1458) were obtained from American Type Culture Collection (Manassas, VA, USA). ..

    Article Title: Bone morphogenetic protein signaling inhibitor improves differentiation and function of 3D muscle construct fabricated using C2C12.
    Article Snippet: Graduate School of Human and Environmental, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto 606-8507, Japan, Department of Chemical Systems Engineering, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan, Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan, Department of Biomedical Engineering, Osaka Institute of Technology, 5-16-1 Omiya, Asahi-ku, Osaka 535-8585, Japan, Department of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan, and Division of Biochemical Engineering, Radioisotope Research Center, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto 606-8507, Japan

    Modification:

    Article Title: Bone morphogenetic protein signaling inhibitor improves differentiation and function of 3D muscle construct fabricated using C2C12.
    Article Snippet: Graduate School of Human and Environmental, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto 606-8507, Japan, Department of Chemical Systems Engineering, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan, Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan, Department of Biomedical Engineering, Osaka Institute of Technology, 5-16-1 Omiya, Asahi-ku, Osaka 535-8585, Japan, Department of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan, and Division of Biochemical Engineering, Radioisotope Research Center, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto 606-8507, Japan



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    Figure 6 Activation of retinoic acid receptor-related orphan receptor-α (RORα) restored the mitochondrial function in glycolytic fibres. (A) Mouse gas- trocnemius (GA) tissues were subjected to immunostaining for MyHC2b (red). Succinate dehydrogenase (SDH) staining was performed in the GA sec- tions of the high-fat diet (HFD)-fed and AAV-RORα-treated mouse model. Tissues were examined by using an automated multimodal tissue analysis system, and representative images are shown. Scale bar: 1 mm (MyHC2b and SDH activity) or 50 μm (RORα). (B) <t>C2C12</t> cells were exposed to differ- entiation medium containing 0.1-mM palmitic acid (PA) conjugated with bovine serum albumin (BSA) and treated with cholesterol sulfate (CH-sulfate) or JC1-40. After 7 days, total RNA was isolated and mRNA level of Myh4 was measured by quantitative real-time PCR (qRT-PCR). ***P < 0.001 versus vehicle (n = 4). (C) Immunostaining was performed with C2C12 cells in the presence of 0.1-mM PA conjugated with BSA at Day 7 of differentiation for staining of MyHC2b (red) and DAPI (blue). Representative images examined by confocal microscopy are shown. Data were obtained from three inde- pendent experiments, and fluorescence intensity was quantified in 10 images of each group by using ImageJ. Scale bar: 25 μm. ***P < 0.001 versus vehicle.
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    Figure 6 Activation of retinoic acid receptor-related orphan receptor-α (RORα) restored the mitochondrial function in glycolytic fibres. (A) Mouse gas- trocnemius (GA) tissues were subjected to immunostaining for MyHC2b (red). Succinate dehydrogenase (SDH) staining was performed in the GA sec- tions of the high-fat diet (HFD)-fed and AAV-RORα-treated mouse model. Tissues were examined by using an automated multimodal tissue analysis system, and representative images are shown. Scale bar: 1 mm (MyHC2b and SDH activity) or 50 μm (RORα). (B) C2C12 cells were exposed to differ- entiation medium containing 0.1-mM palmitic acid (PA) conjugated with bovine serum albumin (BSA) and treated with cholesterol sulfate (CH-sulfate) or JC1-40. After 7 days, total RNA was isolated and mRNA level of Myh4 was measured by quantitative real-time PCR (qRT-PCR). ***P < 0.001 versus vehicle (n = 4). (C) Immunostaining was performed with C2C12 cells in the presence of 0.1-mM PA conjugated with BSA at Day 7 of differentiation for staining of MyHC2b (red) and DAPI (blue). Representative images examined by confocal microscopy are shown. Data were obtained from three inde- pendent experiments, and fluorescence intensity was quantified in 10 images of each group by using ImageJ. Scale bar: 25 μm. ***P < 0.001 versus vehicle.

    Journal: Journal of cachexia, sarcopenia and muscle

    Article Title: RORα-GABP-TFAM axis alleviates myosteatosis with fatty atrophy through reinforcement of mitochondrial capacity.

    doi: 10.1002/jcsm.13432

    Figure Lengend Snippet: Figure 6 Activation of retinoic acid receptor-related orphan receptor-α (RORα) restored the mitochondrial function in glycolytic fibres. (A) Mouse gas- trocnemius (GA) tissues were subjected to immunostaining for MyHC2b (red). Succinate dehydrogenase (SDH) staining was performed in the GA sec- tions of the high-fat diet (HFD)-fed and AAV-RORα-treated mouse model. Tissues were examined by using an automated multimodal tissue analysis system, and representative images are shown. Scale bar: 1 mm (MyHC2b and SDH activity) or 50 μm (RORα). (B) C2C12 cells were exposed to differ- entiation medium containing 0.1-mM palmitic acid (PA) conjugated with bovine serum albumin (BSA) and treated with cholesterol sulfate (CH-sulfate) or JC1-40. After 7 days, total RNA was isolated and mRNA level of Myh4 was measured by quantitative real-time PCR (qRT-PCR). ***P < 0.001 versus vehicle (n = 4). (C) Immunostaining was performed with C2C12 cells in the presence of 0.1-mM PA conjugated with BSA at Day 7 of differentiation for staining of MyHC2b (red) and DAPI (blue). Representative images examined by confocal microscopy are shown. Data were obtained from three inde- pendent experiments, and fluorescence intensity was quantified in 10 images of each group by using ImageJ. Scale bar: 25 μm. ***P < 0.001 versus vehicle.

    Article Snippet: Cell lines and cell culture Mouse myoblast C2C12 cells (CRL-1772, ATCC, Manassas, VA, USA) were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Hyclone, Logan, UT, USA) containing 10% foetal bovine serum (FBS).

    Techniques: Activation Assay, Immunostaining, Staining, Activity Assay, Isolation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Confocal Microscopy